4201374 复 4201315
1:17 PM (3 hours ago)
we have successfully used NgAgo in HeLa cells following a protocol where we used 700ng NgAgo plasmid and 500 ng gDNA (non purified, heat inactivated PNK). We confirmed knockout of GFP from a plasmid and could see the effect even after 96h. About 40% reduction in Western blot could be seen although there is scope for more optimization.
No way to know yet actually since we have done this once just to check if there is a reduction in gfp which we observed both by facs and western. We are currently sequencing the products to rule out Rnai if any. Will let you know if that's confirmed
Dr. Debojyoti Chakraborty
Room 129, CSIR-Institute of Genomics and Integrative Biology
New Delhi - 110025
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