主题:【整理】中国开发出替代CAS9的新型基因编程技术,诺奖级 -- 尖石

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2016-06-30 02:35:35
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陈王奋起挥黄钺
陈王奋起挥黄钺`27847`http://bbs.anjia.com/UploadFace/149060_200910171856426339.jpg`70`29368`95727`800443`正九品上:儒林郎|仁勇校尉`2008-09-20 06:57:55`
韩本人在北大演讲透露,有20多家能重复 4

他还进一步说,原来的版本是初级版,实验手法比较复杂,需要较高的实验技巧,他又特别研发了升级版2.0版,以及smart版,据说很快会对社会公开。

今天我在知乎还看到一个印度实验室声称能够重复了。

Debojyoti Chakraborty

1:17 PM (3 hours ago)

Hello everyone,

we have successfully used NgAgo in HeLa cells following a protocol where we used 700ng NgAgo plasmid and 500 ng gDNA (non purified, heat inactivated PNK). We confirmed knockout of GFP from a plasmid and could see the effect even after 96h. About 40% reduction in Western blot could be seen although there is scope for more optimization.

cheers

debo

作者:John Han

链接:https://www.zhihu.com/question/46968937/answer/108492103

Hi,

No way to know yet actually since we have done this once just to check if there is a reduction in gfp which we observed both by facs and western. We are currently sequencing the products to rule out Rnai if any. Will let you know if that's confirmed

Best

Debo

Dr. Debojyoti Chakraborty

Room 129, CSIR-Institute of Genomics and Integrative Biology

Mathura Road,

New Delhi - 110025


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2016-06-30 02:35:35

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