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主题：【求助】猫猫去世 培养保存猫猫的活细胞 HELP 上海 -- hulihuo
家园 【求助】猫猫去世 培养保存猫猫的活细胞 HELP 上海 我在上海 我的猫猫去世了 因为国内还没法做猫猫的克隆，所以我想培养并保存猫猫的活细胞，请问有谁可以做吗？
我的EMAIL [email protected]
我的电话：18918968130
家园 准备转移至-20度的冰柜里,请问用什么包裹猫猫来保护遗体 准备转移至-20度的冰柜里,请问用什么包裹猫猫来保护遗体比较好,谢啦。
家园 大概是来不及了 如果放在4度的话，或许可以分出一些活的细胞。冻了10天，大概什么也拿不到了。
克隆一只猫的费用少说也要500万以上吧。
家园 死了几天了？ 解决方法有两个：
1，刚死掉可以直接扔冰箱里，然后等着以后有人接活的时候做SCNT。有人从冰箱里找出一个十几年前挂掉的jerry,做SCNT成功了。估计jerry的死对头tom也能行。
2.建系，毛囊干细胞最好。扩起来扔液氮罐里，等有人接活了做iPS然后打四倍体互补克隆。
PS，这个东西技术上没什么难度，毕竟中国在人和小鼠上都有相当的经验。只是没人有那个闲钱和闲心去把成熟的技术应用到猫身上。
- 复 死了几天了？
家园 算上今天的话,15天了 体细胞核移植(somatic cell nuclear transfer，SCNT)是这个吗?太好了,冷炎3兄,国内可有能做这种活的朋友引荐的吗?多谢啦
家园 放在什么里头的？-20冷冻？4度冷藏 15天如果保存方式不合理的话就不行了。我只知道有人做人的，不知道有人做猫的。下面是做猪的
Cell Reprogram. 2011 Oct 26. [Epub ahead of print]
Histone Deacetylase Inhibitor Significantly Improved the Cloning Efficiency of Porcine Somatic Cell Nuclear Transfer Embryos.
Huang Y, Tang X, Xie W, Zhou Y, Li D, Yao C, Zhou Y, Zhu J, Lai L, Ouyang H, Pang D.
Source
Jilin Province Key Laboratory of Animal Embryo Engineering, College of Animal Science and Veterinary Medicine, Jilin University , Changchun, People's Republic of China .
Abstract
Abstract Valproic acid (VPA), a histone deacetylase inbibitor, has been shown to generate inducible pluripotent stem (iPS) cells from mouse and human fibroblasts with a significant higher efficiency. Because successful cloning by somatic cell nuclear transfer (SCNT) undergoes a full reprogramming process in which the epigenetic state of a differentiated donor nuclear is converted into an embryonic totipotent state, we speculated that VPA would be useful in promoting cloning efficiency. Therefore, in the present study, we examined whether VPA can promote the developmental competence of SCNT embryos by improving the reprogramming state of donor nucleus. Here we report that 1mM VPA for 14 to 16h following activation significantly increased the rate of blastocyst formation of porcine SCNT embryos constructed from Landrace fetal fibroblast cells compared to the control (31.8 vs. 11.4%). However, we found that the acetylation level of Histone H3 lysine 14 and Histone H4 lysine 5 and expression level of Oct4, Sox2, and Klf4 was not significantly changed between VPA-treated and -untreated groups at the blastocyst stage. The SCNT embryos were transferred to 38 surrogates, and the cloning efficiency in the treated group was significantly improved compared with the control group. Taken together, we have demonstrated that VPA can improve both in vitro and in vivo development competence of porcine SCNT embryos.
家园 这个可以吗,我也有兴趣,顶一下